Special Stain NoteBook Research Paper Example | Topics and Well Written Essays - 3500 words

Special Stain NoteBook - Research Paper Example Again, the tissue is rinsed in the stain with cold water and finally, dipped in ethanol fixate, then in xylene for forty-five seconds (Yakovlev, 2009). Procedure (Steps): Bouin’s solution, an inorganic oxide, is used in microwave for one minute and is allowed to stand for fifteen minutes. Subsequently, for five minutes, the stain is washed in tap water, followed by the application of one drop of hematoxylin and is allowed to stand for ten minutes. Correspondingly, it is again washed in tap water for five minutes and then rinse in distilled water. In the following step, the stain is dipped in biebrich scarlet solution for five minutes and then rinsed in distilled water. Then phosphomolybdic or phosphotungstic is applied with a resting time of ten minutes required to discard it. Sixth, then transferred to aniline blue solution, and is kept for five minutes. The stain is then rinsed in distilled water. Again one percent of acetic acid is applied on the specimen and rinsed in distilled water. Finally, it is dehydrated and cleared (Media Lab Incorporated, 2015). Procedure (Steps): At the initial step, sections of the tissue is deparaffinized and hydrated and then suspended in distilled water. In the second step, 250 ml of iron stain solution is added to the specimen and dipped for thirty minutes. Then the specimen needs to be washed for three times in distilled water. Again it needs to be stained with 250 ml nuclear fast red solution for five minutes and then rinsed in distilled water. In the third step, the specimen needs to be dehydrated through 95% of alcohol for two changes, followed by the final and the last process to clear it in xylene solution for three changes and for two times, before covering the slip by using permanent mounting medium (Biogenex, 2015). Procedure (Steps): First, deparaffinize sections of the liver and hydrate it to deionized water. Second, oxidize the liver section in 1% potassium